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furthermore, CR also reduced the expression of proapoptotic genes Bcl-xS/Bcl-xL, mitochondrial translocation of BAX, release of cytochrome c into cytosol, and caspase-9 activation (Niemann et al., 2010)

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7.3 Ongoing challenges and future directions The translation of ferroptosis-targeted therapies faces several significant challenges that require careful consideration: 7.3.1 Nanocarrier delivery challenges While nanocarriers offer promising targeting capabilities, they face substantial translational hurdles including potential immunogenicity, nebulizer compatibility and stability, batch-to-batch consistency in manufacturing, and difficulties achieving uniform deep lung penetration and cellular uptake across different airway regions and cell types