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The concept of IMAC was developed by Porath and co-workers in 1975, through the exploitation of the affinity between proteins and heavy metal ions (Zn 2 + , Cu 2 + , Ni 2 + ), in particularly zinc and copper metal which strongly adsorb polypeptides containing histidine and cysteine residues in aqueous solutions (Porath et al., 1975)

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