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Cells were incubated with 30 μM dihydroethidium (DHE) probe (Invitrogen, D11347) for 1 hour in the dark at 37 C°
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Hemoglobinopathies are defined by an imbalance of globin chains and inadequate erythropoiesis, resulting in premature oxidative damage and the release of structurally impaired red blood cells with a reduced lifespan (33, 69)
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