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Then the gene expression was quantified dependent on the LightCycler480 PCR system (Roche) by using 2×SYBR Green qPCR Master Mix (biotool, #B21203)
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Nanoencapsulation, ultrasonic and microwave‐based approaches, and supercritical fluid extraction all offer auspicious means to improve extraction yields, selectivity, and bioavailability
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