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Keywords: growth hormone, insulin-like growth factor-I, intra-portal insulin, acromegaly, adult growth hormone deficiency, hepatic growth hormone sensitivity Introduction The growth hormone (GH)-insulin-like growth factor I (IGF-I) axis plays a critical role in promoting linear growth in children (1), whereas in adults, its role is primarily of metabolic relevance (2)

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The following day, the cultures were diluted 1:100 into 1L LB broth and allowed to grow at 37°C while shaking at 280 rpm until the cultures reached an optical density at 600 nm (OD 600 ) of 0.6, measured by NanoDrop One (Thermo Fisher Scientific, Cat# 13–400-518)