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Extracellular Acidification Rate (ECAR) measurement BMDMs were seeded in a Cell-Tak-coated Seahorse XFp Cell Culture plate (40000 cells per well) in Agilent Seahorse XF Base Medium supplemented with 2mM L-Glutamine
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However, with longer term ethanol exposure, the hyperactivation of PARP-1 occurs, which may cause pro-apoptotic activities (14)
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