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In line with the mapping of the ligand, the sst receptors are widely expressed in the rodent brain with the following regional pattern: all layers of the cerebral cortex (sst 1 , sst 2a/b , sst 3 , sst 4 ), bed nucleus of the stria terminalis (sst 1 , sst 2a, b , sst 4 ), hippocampus (sst 1 , sst 2a, b , sst 3 , sst 4 ), basolateral amygdaloid nucleus (sst 1 , sst 2a/b , sst 3 , sst 4 ), medial amygdaloid nucleus (sst 1 , sst 2 , sst 3 ), ventromedial hypothalamic nucleus (sst 1 , sst 2 , sst 3 ), dorsomedial hypothalamic nucleus (sst 1 , sst 3 ), paraventricular nucleus (sst 2a , sst 3 ) and arcuate nucleus of the hypothalamus (sst 1 , sst 2a , sst 3 , sst 4 ), substantia nigra (sst 1 , sst 2a/b , sst 3 ), dorsal raphe nucleus (sst 1 , sst 2 , sst 3 ), locus coeruleus (sst 2 , sst 3 ), granular layer of the cerebellum (sst 1 , sst 2b , sst 3 , sst 4 , sst 5 ), dorsal motor nucleus of the vagus nerve (sst 2a, b , sst 4 , sst 5 ) and nucleus of the solitary tract (sst 1 , sst 2 , sst 3 ) (Schindler and Humphrey, 1999

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A specific standard for each gene allows for absolute quantification of the gene in copy numbers, which can then be expressed as ng per μg of RNA