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Wnt/β-catenin pathway as a biomarker in the diagnosis of tumour-related diseases Wnt/β-catenin pathway in the cell-free DNA (cfDNA) test for CRC The traditional diagnosis of CRC has many limitations, 243,244,245 such as low sensitivity, poor specificity, and painful and traumatic procedures

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258 However, contrary to this, trials such as the IMpower-010 and KEYNOTE-091 trials support the approach of upfront surgery followed by adjuvant chemotherapy and one year of adjuvant immunotherapy, which also holds promise in terms of curative potential 228,246 (Fig