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The GST protein and GST fusion protein were incubated with GST MAGIC beads in incubating buffer (50 mM HEPES pH 7.5, 50 mM NaCl, 10 mM EDTA, 0.2% (v/v) Triton X-100, 10% (v/v) Glycerol, 2 mM DTT, 1 × protease inhibitor, 1 × PMSF) at 4 °C for 1.5 h
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