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Once the cells attained 80% confluency, they were treated with either 20 µM CER 16:0 (Enzo Life Sciences, BML-SL115), 25 μM 2-OE (Invitrogen, 0383), or EtOH vehicle for 6 h, and either collected for protein analysis by WB, or fixed with 4% paraformaldehyde for IF
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