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Each of these receptor peptides were made into individual stock solutions with a concentration of 1 mg/mL in pH 7.00 phosphate-buffered saline solution (Fischer Scientific, Hampton, NH, USA)
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Fusion of GRX1 to roGFP2 leads to a permanent increase of the local GRX concentration around roGFP and hence this kinetic coupling further increases the likelihood of interaction between roGFP and the fused GRX1 compared to other non-specific interactions with other oxidoreductases (Gutscher et al., 2008
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