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Osteoblast differentiation After the POBs became confluent, they were pretreated with or without Cpd33 (1 μM) and then cultured in osteoblast differentiation medium (α-MEM containing 5% FBS, 10 mM β-glycerophosphate (β-GP), and 50 μg/ml ascorbic acid) in the presence or absence of LPS (100 ng/ml)