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This allowed us to build the γ-Glu-FA topology (available at using the CHARMM36 force field 43 format (named using a three-letter name CG9)
metabolic
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Cells at 20 ~ 30% confluency were transfected with 3 μg pcDNA3.3-dsRed/Sabin1-P1 or pcDNA3.3-dsRed/Mahoney-P1 formulated with Lipo3000 transfection reagent (Invitrogen), and 2 μg pcDNA3.3-T7 and 2 μg pT7-Replicon-eGFP were co-transfected the next day
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