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Features of Glutathione Agarose: • Slurry —crosslinked 6% beaded agarose, slurried in water with sodium azide • High capacity —binds at least 40 mg of purified recombinant GST protein per milliliter of resin • High yield and purity —consistently purifies at least 10 mg of GST-tagged protein per milliliter of resin with greater than 90% purity • Cost-effective —resin is economically priced and can be reused at least five times without reduction in binding capacity and purification performance • Versatile —works well to purify GST-fusion proteins from bacterial lysates or use with pre-purified GST-tagged proteins to pull down protein interactions • Compatible —validated and effective for use with Thermo Scientific Cell Lysis Reagents to extract and purify from bacterial or mammalian cell cultures The high-quality support consists of glutathione that has been immobilized by its central sulfhydryl group via a 12-atom spacer arm to crosslinked 6% beaded agarose
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