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The DNA sequence for the ARE was obtained from the National Center for Biotechnology Information (GenBank: JQ858521.1), and a synthetic ARE polynucleotide was inserted into the Hin dΙΙΙ site in multicloning site of pGL4.26 using the In-Fusion HD cloning kit (Takara)
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Monocyte-derived macrophages matured under prolonged hypoxia transcriptionally up-regulate HIF-1α mRNA
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