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a “high stringency” is defined herein as being a hybridization and/or wash carried out in 0.1 ⁇ SSC buffer, 0.1% (w/v) SDS, or lower salt concentration, and at a temperature of at least 65° C., or equivalent conditions
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CONCLUSION: The GSIS occurs independently of a detectable increase in beta cell cytosolic or mitochondrial H2O2 levels
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