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The model collected over time according to the previous design considering the physiological stage of calf growth or weeks of collection (time, 0, 40, and 60 days), diets (Control, SeVitE, SeVitEFe), and the interaction between time and treatment using PROC MIXED from SAS, according to the following model: Where Y ijklm is the dependent variable value, μ is the overall mean, D i is the fixed effect of diet l ( i = 1–3), A j (D) k , is the random effect of the animal within of diet, ε ijk is the residual error