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An affinity tool for the isolation of endogenous active mTORC1 from various cellular sources - ScienceDirect
318 (6), G1042–G1053
Type_Full Kit
Style:Grapefruit
RT-PCR and construction of expression vectors cDNA was reverse transcribed from total RNA, and three PCR fragments (K17, S1, and S4) were amplified using AmpliTaq Gold DNA polymerase (Life Technologies) using the following primers: K17 (F 5′AT GGATCC CTCTCCAGCCCTTCTCCT-3′ ( Bam HI site underlined), R 5′AT GAATTC TCAGGCAAGGAAGCATG-3′ ( Eco RI site underlined)
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