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The time at which NEM was added (0–60 min) and temperature (at 4 °C, 25 °C and 50 °C) dependent stability of GSH were evaluated by assessing the autoxidation of 200 µM and 2000 µM GSH to GSSG
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In contrast to α1(L275C) with β1(A316C) and α1(L425C) with β1(L365C), α1(L275C) did not crosslink with β1(L365C), and α1(L425C) did not crosslink with β1(A316C), owing to their long spatial distance
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