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[DOI] [PMC free article] [PubMed] [Google Scholar] 159.Cau J, Favreau F, Tillement JP, Lerman LO, Hauet T, Goujon JM
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For SDS–PAGE, 80 μL of each fraction were mixed with 40 μL of 2× sample buffer (62.5 mM Tris-HCl, pH 6.0, 8 M urea, 2% SDS, 4% β-mercaptoethanol), heated at 90°C for 2 min, and 25 μL (corresponding to ∼3% of each fraction) were resolved on 8% polyacrylamide gels
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