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The last GSH synthetic process is the one depending on the conversion of the oxidized dimer GSSG to 2 reduced GSH molecules in cells by glutathione reductase (GR), an ubiquitous enzyme of the family of disulfide reductases, in the presence of NADPH and flavin adenine dinucleotide -FAD-
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We therefore concluded that BR-GBDΔΔ, which lacks both BR and the CRIB domain, shows no recruitment to GUV-bound SemD Rhod
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