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Reactions were stopped after 1 h by addition of 950 µl of 0.4 M glycine-NaOH (pH 8.0), samples were vortexed and spun down briefly, and the supernatant transferred to a round-bottomed glass tube
IV amino acids
CT:60 ct
Furthermore, it increased itaconic acid production through inhibition of immune response gene 1 (IRG1) and succinate dehydrogenase (SDH) activity—an enzyme responsible for the negative regulation of HIF-1α/NLRP3 signaling and, consequently, for the polarization of M1 to M2 macrophages [281]
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