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Both GshF - and mito-GshF expression prevented BSO-induced cell death up to millimolar amounts of drug treatment in a panel of human and mouse cell lines (Fig
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Since we expect the native-like structures of the fusion proteins to be preserved in the solubilization experiments that we tested, which also bypassed the refolding step (and, saved time), we wanted to ascertain the intactness of the secondary structure
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