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Then, the cells were subjected to immunofluorescence staining for α-SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 °C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

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There is evidence that the K channel provides the route for one or two of the protons used in reduction of O 2 to H 2 O, while the D channel provides the route for two or three of the substrate protons, and all four translocated protons [134]