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a mutant yeast strain having at least two mutations selected from the following groupings, which may overlap: i) mutation in a gene or genes encoding components of the mitochondrial respiratory chain or nuclear genes encoding proteins that maintain the integrity of the mitochondrial genome or mutation or deletion of the mitochondrial genome;ii) mutation in a gene or genes affecting intracellular levels of NAD(P)H and NAD(P) +

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In brief, chemical standard libraries of 149 metabolites in seven pooled libraries were prepared and serially diluted in high-performance liquid chromatography (HPLC)-grade water from in a dilution series from 5 mM to 1 µM to generate external standard pools, which are used for calibration of isotopically labelled internal standards and to quantify concentrations of metabolites for which internal standards were not available