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GAPDH activity assay For GAPDH activity assay, 300,000 A549 cells were separately seeded per well of a 6-well plate in quadruplicates per condition, with conditions being: 1 µM, 5 µM, 10 µM KA treatment or DMSO control
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This upregulation of CYP2E1 explains, in part, the metabolic tolerance to alcohol and the cross-tolerance to other drugs that are also substrates of CYP2E1, such as pentobarbital and diazepam, in people with AUD (90)
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