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Using it and then describing the outcome as what IGF-1 does inverts the logic of the experiment
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Protein samples (20 μg) were separated on NuPage ® Novex ® 4%–12% Bis-Tris gels (Life Technologies, Carlsbad, CA, USA), then transferred to PVDF membranes (Rockford, IL, USA), which were blocked with non-fat milk and incubated with primary antibodies (OXPHOS complexes
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