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The standard external solution for ionic current recordings contained (in mM) 120 NaCl, 5 KCl, 20 TEA chloride, 10 D-glucose, 10 Tricine, and 2.8/5.8 CaCl 2 or 10 HEPES and 2.3/4.5 CaCl 2 (= 2/4 mM free Ca 2+
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The surgeon will then remove the equipment, and insert gas into the abdomen to check the integrity of the anastomoses alongside inspecting for any bleeding in the operative field
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