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Moreover, dietary methionine restriction impairs human CAR-T cell efficacy against lung squamous cell carcinoma in mice (137)
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Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR) for TGF-β1 mRNA Total RNA was extracted from frozen lung tissues with TRIzol reagent (TaKaRa Biotechnology Co., Ltd., Dalian, China) and was reverse-transcribed using a reverse transcription kit (TaKaRa Biotechnology Co., Ltd., Dalian, China)
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