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In this case, we were able to identify 1,403 phosphopeptides from 100 μg of digest via TiO 2 SPE alone
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The DCF was quantified using a standard curve as previously reported (Rodrigues et al., 2005) with a few modifications ( 2.7 Malondialdehyde (MDA) lipid peroxidation assay 200 μL of cytosol samples were mixed with 1 mL of acetic acid (20%) then 200 μL of 8% sodium dodecyl sulphate
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