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Firstly, the high-level expression of the two antigens was confirmed by FACS that the antigens on 1E4 Raji cells or 3E3 BT474 cells in 100 μL could be saturated by 0.1 μg/mL Rtx or Hct (Fig
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Finally, EVs were obtained by centrifugation at 100,000 × g , followed by filtration through 0.22 μm or 0.45 μm pore-size membranes to enhance purity
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