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Each animal was placed in the center of the maze, facing one of the open arms, and allowed to freely explore the maze for 5 minutes

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Similarly, if Cu 2+ weakly bound to the sulfur atom of Met35 is the recipient of an electron from this S-atom to form the reduced Cu + (see above for discussion of a relatively weaker electron pair interaction with the S-atom protons in a polypeptide with α-helical structure), this latter moiety can lead to the highly reactive hydroxyl free radical by Fenton chemistry as noted, initiating lipid peroxidation