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Then, primary antibody binding was detected after incubating sections with a horseradish peroxidase conjugated-secondary antibody (polyclonal anti-goat or anti-rabbit IgG HRP, R&D System, Minneapolis, MN, USA) for 2.5 h, followed by a 20 min exposure to the HRP substrate 3-amino-9-ethylcarbazole (AEC)

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