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The controlled conditions of clinical research also differ from real-world use

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A number of other synthetic approaches have since been developed including a large-scale production method involving the reaction of γ-benzyl glutamate in the presence of trityl chloride and ethylamine [339 g kg -1 ] [29] and a two-step approach involving initial dehydration of L-glutamic acid to L-pyrrolidone carboxylic acid followed by ring opening in the presence of ethylamine to yield theanine [374 g kg -1 ] [30]